How do microbial interactions affect antibiotic susceptibility?

Interview with Lauren Radlinski and Brian Conlon on their latest Plos Biology paper

Go to the profile of Ben Libberton
Dec 11, 2017
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New research from the University of North Carolina at Chapel Hill shows that different strains of Pseudomonas aeruginosa can affect the susceptibility of Staphylococcus aureus to a range of different antibiotics. Depending on the antibiotic and the strain, Ps. aeruginosa can either enhance killing or provide protection against the antibiotics tested. Given the high treatment failures, especially in chronic infections such as cystic fibrosis where Ps. aeruginosa and S. aureus can be found together, this work highlights the importance of understanding microbial interactions when administering antibiotics.


Chronic coinfections of Staphylococcus aureus and Pseudomonas aeruginosa frequently fail to respond to antibiotic treatment, leading to significant patient morbidity and mortality. Currently, the impact of interspecies interaction on Saureus antibiotic susceptibility remains poorly understood. In this study, we utilize a panel of Paeruginosa burn wound and cystic fibrosis (CF) lung isolates to demonstrate that Paeruginosa alters Saureus susceptibility to bactericidal antibiotics in a variable, strain-dependent manner and further identify 3 independent interactions responsible for antagonizing or potentiating antibiotic activity against Saureus. We find that Paeruginosa LasA endopeptidase potentiates lysis of Saureus by vancomycin, rhamnolipids facilitate proton-motive force-independent tobramycin uptake, and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) induces multidrug tolerance in Saureus through respiratory inhibition and reduction of cellular ATP. We find that the production of each of these factors varies between clinical isolates and corresponds to the capacity of each isolate to alter Saureus antibiotic susceptibility. Furthermore, we demonstrate that vancomycin treatment of a Saureus mouse burn infection is potentiated by the presence of a LasA-producing Paeruginosa population. These findings demonstrate that antibiotic susceptibility is complex and dependent not only upon the genotype of the pathogen being targeted, but also on interactions with other microorganisms in the infection environment. Consideration of these interactions will improve the treatment of polymicrobial infections.

Author summary

Accurate prediction of antimicrobial efficacy is essential for successful treatment of a bacterial infection. While many studies have considered the impacts of genetically encoded mechanisms of resistance, nongenetic determinants of antibiotic susceptibility during infection remain poorly understood. Here we show that a single interspecies interaction between 2 human pathogens, Saureus and Paeruginosa, can completely transform the antibiotic susceptibility profile of Saureus. Through multiple distinct mechanisms, Paeruginosa can antagonize or potentiate the efficacy of multiple classes of antibiotics against Saureus. We identify the exoproducts responsible for altering Saureus susceptibility to antibiotic killing, and furthermore demonstrate that these compounds are produced at varying levels in Paeruginosa clinical isolates, with dramatic repercussions for Saureus antibiotic susceptibility. Finally, we use a mouse model of Paeruginosa–Saureus coinfection to demonstrate that the presence of Paeruginosasignificantly alters the outcome of Saureus antibiotic therapy in a host. These findings indicate that the efficacy of antibiotic treatment in polymicrobial infection is determined at the community level, with interspecies interaction playing an important and previously unappreciated role.


Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus  Radlinski L, Rowe SE, Kartchner LB, Maile R, Cairns BA, et al. (2017) Pseudomonas aeruginosa exoproducts determine antibiotic efficacy against Staphylococcus aureus. PLOS Biology 15(11): e2003981.

Go to the profile of Ben Libberton

Ben Libberton

Communications Officer, MAX IV Laboratory

I'm a Communications Officer at MAX IV Laboratory in Lund, Sweden, formally a Postdoc in the biofilm field. I'm interested in how bacteria cause disease and look to technology to produce novel tools to study and ultimately prevent infection. Part of my current role is to find ways to use synchrotron radiation to study microorganisms.


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Iqbal Ahmad 2 months ago


Go to the profile of Iqbal Ahmad
Iqbal Ahmad 2 months ago

Very intestine findings. It is well expected but explored probably for the first time.

Go to the profile of Iqbal Ahmad
Iqbal Ahmad 2 months ago

very interesting findings.Congratulation.