Antibiotic resistance and macromolecules
Macromolecules have an antibacterial activity that can possibly combat the problem of antibiotic resistance. This is one of the best weapons leading the fight against antibiotic resistance. Talking about the process, two macromolecules have constantly been targeting bacterial membrane and even potentiating the conventional antibiotics. These macromolecules have been found helpful in demonstrating a novel approach in order to treat topical Gram-negative bacterial infections. This has been noted according to a recent study published in PLoS One.
The study’s authors noted that the bacterial biofilms are basically the cause of recurring infections and bacteria present within the biofilms are metabolically dormant. This renders conventional antibiotics to help the fight against Gram-negative bacteria ineffective. At the same time, the dormant bacterial population works on reactivating post-antibiotic treatment. However, new strategies will be needed for the treatment of chronic bacterial infections.
Disrupting the bacterial membrane is one of the many strategies that can be adopted. This has been demonstrated in the previous studies with respect to the cationic and hydrophobic macromolecules. These macromolecules even help in delaying or preventing the development of bacterial resistance. Because of these characteristics, the authors of the current study note that it could be used to disrupt Gram-negative bacterial biofilms. For this, the authors have performed a series of experiments with the aim of evaluating the activity of two macromolecules to fight several Gram-negative bacterial species. Along with this, the antibacterial activity of conventional antibiotics was even evaluated, alone and in combination with the molecules.
Talking about the experiments, vitro and vivo experiments were performed. In vitro studies, activities undertaken were against antibiotic-tolerant, dormant, and actively growing bacteria. It was compared with conventional antibiotics. In this case, membrane disruption, biofilm disruption, Antibiotic Potentiation, and resistance were noted. On the other hand, in vivo studies, safety and efficacy were noted.
Biofilm disruption being the most important point, it was worked upon with the help of laser scanning microscopy. With this, the authors noted the disruption of E. coli. These findings suggested the molecules’ membrane-active mechanism of action. These revelations, in this case, are as important as an electric motor for a manufacturing company. So, the details and valuable and there is a lot of support generated from other sections.
On a concluding note, the authors noted that a combination therapy with such membrane-active macromolecules and even conventional antibiotics had shown great therapeutic potential for issues related to topical Gram-negative bacterial infections.