Tools and Techniques for Microbiome Analysis
Panel discussion presented by the SF Microbiome Meetup and Illumina Accelerator.
The San Francisco Microbiome Meetup hosted a networking event at the Illumina Accelerator featuring a panel discussion on tools and techniques for analysis. The panel consisted of Nick Conley of Epibiome, David Hanzel of Naked Biome, Alberto Acedo of Biome Makers, Elisabeth Bik of uBiome, and Amanda Cashin of Illumina Accelerator as moderator. The following were some of the themes covered:
Standardization and research protocols
A difficulty facing industry and academia alike is the ability to analyze findings across labs. Given the already inherent challenges of studying microbiota - i.e., the dynamic and variable nature of the ecosystems - a key is standardization through rigorously and meticulously defined protocols. Inclusion of even seemingly insignificant details helps intra-lab analyses to be conducted. And this should be one of the main goals for any lab. The replicability for inter-lab analyses will in turn become easier with each defined protocol in place.
Robotics and automation
Along the lines of standardization, the panel was particularly excited about the dawning age of robotics and automation in the lab bench setting. Not only will robotics allow flexibility of working location (Nick encourages the beach) but it also allows more rapid, consistent, and replicable testing.
A consistent pain point for the panelists is the black box methodology that most labs use when defining OTUs (Operational Taxonomic Unit). The panelists would encourage companies and labs to have more clearly defined OTUs with genomic data backing (when access is possible). This will allow groups to compare and validate test findings more easily.
Unfortunately, the panel was not able to go into detail on portability, but I personally believe this is one of the main areas for tools growth. While the MinION Sequencer has a high error rate and higher cost per sequence, the future of sequencing is portable. Being able to analyze samples at point of collection will enable the mass proliferation of microbiome studies. Hopefully a toilet with a built in sequencer isn’t too distant a dream!
To join the SF Microbiome Meetup, see our LinkedIn group.
To reach out to George, go to revitalbiome.com.